guystarthreepwood
guystarthreepwood t1_j8nxq4g wrote
Reply to comment by clebo99 in Questions raised about Baltimore’s $4.5 million study to retime its traffic signals | Baltimore Brew by bmore
Right? I'm sure the result is going to be "holy fuck this shit is BAD" I just remember 2 or 3 times when I was commuting by bus from UMMC to Hopkins (2017-2020) when out of nowhere for 2 weeks the entire thing gridlocked. The trip would take twice as long for no apparent reason. I always joked that someone must have been trying to "fix" the lights...
guystarthreepwood t1_j96tye4 wrote
Reply to comment by Appropriate_Banana in Pharmacological vitamin C inhibits mTOR signaling and tumor growth by degrading Rictor and inducing HMOX1 expression (Feb 2023) by basmwklz
Preface: it has been a minute since I studied these systems in detail, this is the jist of it. Vitamine c is a redox active molecule existing in an equilibrium between reduced and oxidized forms tending to favor the reduced form. Ascorbic acid (reduced vitamin c) gets oxidized by taking on a single electron from a radical (molecule with an unpaired electron) to form oxidized vitamin c. If it is exposed to oxygen or other oxidants for long periods of time it will exist largely in the oxidized form and end up being a prooxidant rather than antioxidant in order to re-establish the equilibrium. Your body is able to reduce oxidized vitamin c via various intracellular antioxidants (glutathione etc) but that initial exposure will produce some ROS.
Also in cell culture, there's often a high concentration of free iron floating around (5mM) which is totally unnatural. The free iron/vitamin c combo is able to create a Fenton reaction and produce huge amounts of ROS.